Gene description for FLOT2
Gene name flotillin 2
Gene symbol FLOT2
Other names/aliases ECS-1
ECS1
ESA
ESA1
M17S1
Species Homo sapiens
 Experiment description of studies that identified FLOT2 in Myeloid leukemia cells

1
Experiment ID879
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562 - control siRNA transfected
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID880
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562 - co-transfected with SphK2 siRNA and empty vector
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID881
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562 - co-transfected with SphK2 siRNA and siRNA-resistant WT SphK2
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID882
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562 - co-transfected with SphK2 siRNA and mutant SphK2 (G248D)
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID883
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID884
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562 - DMS treated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID885
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29955024   
OrganismHomo sapiens
Experiment descriptionEssential Role of Sphingosine Kinase 2 in the Regulation of Cargo Contents in the Exosomes from K562 Cells
AuthorsMohamed NNI, Okada T, Kajimoto T, Nakamura SI.
Journal name Kobe J Med Sci
Publication year2018
SampleMyeloid leukemia cells
Sample nameK562 - HACPT treated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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